目的 研究薯蓣(Dioscorea opposite Thunb.)叶腋间珠芽(零余子)的化学成分。方法 采用硅胶、Toyopearl HW-40C、Sephadex LH-20、MCI gel CHP-20及ODS等柱色谱技术结合半制备液相色谱进行分离纯化,根据其理化性质、核磁共振(NMR)谱、质谱鉴定所得化合物结构。结果 从零余子中分离得到16个化合物,分别鉴定为吐叶醇(1)、长寿花糖苷(2)、苯乙醇-8-O-β-D-葡萄糖苷(3)、artselaeroside A(4)、benzyl-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside(5)、seguinoside E(6)、dioscorolide A(7)、芹菜素(8)、5,7-dihydroxy-2-[2-(4-hydroxyphenyl)ethyl]chromone(9)、2,7-dihydroxy-4,6-dimethoxyphenanthrene(10)、对羟基苯乙酮(11)、2-hydroxy-3,5,7-trimethoxy-9,10-dihydrophenanthrene(12)、杜鹃醇(13)、3-甲氧基-4-羟基-苯乙醇(14)、3,5-二甲氧基-4-羟基-苯乙醇(15)、4-(3,4-二羟基苯基)-2-丁酮(16)。结论 化合物2～6、9、11～16为首次从薯蓣属中分离得到,其他化合物为首次从薯蓣中分离得到。
Abstract
OBJECTIVE To investigate the chemical constituents from the bulbils of Dioscorea opposite Thunb. METHODS The chemical constituents were isolated and identified by various separation techniques such as silica gel, Toyopreal HW-40C,Sephadex LH-20,MCI gel CHP-20, ODS and preparative HPLC.Their structures were elucidated by analysis of physicochemical properties and spectroscopic evidence. RESULTS Sixteen compounds were isolated and identified as vomifoliol(1), roseoside(2),phenethyl-8-O-β-D-glucopyranoside(3), artselaeroside A(4),benzyl-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside(5), seguinoside E(6),dioscorolide A(7),apigenin(8),5,7-dihydroxy-2-[2-(4-hydroxyphenyl)ethyl]chromone(9), 2,7-dihydroxy-4,6-dimethoxyphenanthrene(10),4-hydroxyacetophenone(11),2-hydroxy-3,5,7-trimethoxy-9,10-dihydrophenanthrene(12), rhododendrol(13), 3-methoxy-4-hydroxyphenethyl alcohol(14), 3,5-dimethoxy-4-hydroxyphenethyl alcohol(15), and 4-(3,4-dihydroxyphenyl)-2-butanone(16). CONCLUSION Compounds 2-6, 9 and 11-16 are isolated from genus Dioscorea for the first time, and the other compounds are isolated from this plant for the first time.
关键词
薯蓣属 /
零余子 /
吐叶醇 /
苯乙醇-8-O-β-D-葡萄糖苷 /
2,7-dihydroxy-4,6-dimethoxyphenanthrene /
杜鹃醇
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Key words
Dioscorea /
bulbil of Dioscorea opposite Thunb. /
vomifoliol /
phenethyl-8-O-β-D-glucopyranoside /
2,7-dihydroxy-4,6-dimethoxyphenanthrene /
rhododendrol
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中图分类号：
R932
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参考文献
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脚注
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基金
国家重点研发计划资助(2017YFC1702800);河南省重大科技专项资助(171100310500)
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